2 edition of Serological tests for the identification of plant viruses found in the catalog.
Serological tests for the identification of plant viruses
Ellen M. Ball
|Statement||(by) Ellen M. Ball ; with sections contributed by A.I.E. Aapola (et al.).|
|Contributions||American Phytopathological Society.|
A lot of methods have been developed to detect plant viruses, such as microscopical observation, serological techniques, molecular methods and so on (Lopez et al., ; Makkouk and Kumari, ; Webster et al., ). Among them, a number of methods for the diagnosis of plant viral diseases are reviewed in the following two sections Cited by: 7. Fundamentals of Plant Virology is an introductory student text covering all of modern plant virology. The author, Dr. R.E.F. Matthews, has written this coursebook based on his classic and comprehensive Plant Virology, Third Edition. Four introductory chapters review properties of viruses and cells and techniques used in their study.
Plant diseases can have an enormous impact on our lives. In a world where total crop failure can quickly lead to human misery and starvation, accurate diagnostics play a key role in keeping plants free from pathogens. In Plant Pathology: Techniques and Protocols, expert researchers provide methods. Quick serological tests can be used in the field or greenhouse for early identification of the pathogen. Several quick tests that were evaluated by the USDA-APHIS-PPQ-CPHST Laboratory are available commercially (Photo 18). Serological methods are generally quick and reliable but suffer from problems with specificity, sensitivity or both.
Ball EM () Serological tests for the identification of plant viruses. The Am Phytopathol Soc Plant Virol Comm, St. Paul, Minn, p 31 Google Scholar Day PR, Dodds JA () Viruses of plant pathogenic by: 1. These viruses do not appear to spread systemically in this host. Serological tests with nonsymptomatic leaves of infected plants are negative. The lesions, though spectacular on some plant leaves, appear to be local lesions and the titer (amount of virus in a given amount of tissue) of virus is low and decreases with time.
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Serological tests for the identification of plant viruses. Paul: American Phytopathological Society, Plant Virology Committee, (OCoLC) Document Type: Book: All Authors / Contributors: Ellen M Ball. Geórgia C.
Anselmo, in Plant Virus–Host Interaction, Molecular techniques for virus detection. Although serology has been used extensively for plant virus identification on a large scale, the use of molecular techniques for plant virus identification and characterization is increasing throughout the world (Lima et al ).
Methods are described for preparing and treating antigens, polyclonal antisera and monoclonal antibodies. Serological principals, methods, step-by-step procedures and applications for detection and differentiation of viral and bacterial plant pathogens are presented.
The manual is intended for students and researchers and comprises 5 sections: Section I. Antigens and. Book: Serological tests for the identification of plant viruses. pp pp. Abstract: In this publication, prepared under the auspices of the Committee on Plant Virology virology Subject Category: Disciplines, Occupations and IndustriesCited by: This book is a comprehensive practical manual containing protocols for identification, characterization and detection of viruses infecting plants.
Various methods based on biological, physico-chemical, serological and nucleic acid properties of the viruses are dealt in detail. Serology and Immunochemistry of Plant Viruses investigates the antigenic properties of plant viruses.
It looks at the practical aspects of plant virus serology, along with the molecular basis of viral antigenicity, antigenic determinants in proteins, the structure of antibodies, virus purification, antiserum production, and the theoretical principles and practical Book Edition: 1.
Serological test, any of several laboratory procedures carried out on a sample of blood serum, the clear liquid that separates from the blood when it is allowed to purpose of such a test is to detect serum antibodies or antibody-like substances that appear specifically in association with certain diseases.
The various types of serological tests include: (1) Flocculation tests, of. Animal virus cultivation is important for 1) identification and diagnosis of pathogenic viruses in clinical specimens, 2) production of vaccines, and 3) basic research studies.
In vivo host sources can be a developing embryo in an embryonated bird’s egg (e.g., chicken, turkey) or a whole animal. Viruses assumed to belong to a new Potyvirus were detected in passionfruit plants with foliar mosaic, leaf curl and fruit malformation that were collected.
Identification of growing virus. The presence of growing virus is usually detected by: Many different types of serological tests are available.
With some assays such as EIA and RIA, one can look specifically for IgM or IgG, whereas with other assays such as CFT and HAI, one can only detect total antibody, which comprises mainly IgG. Serological Identification of Plant Viruses and Serological Diagnosis of Virus Diseases of Plants Annual Review of Microbiology Vol.
(Volume publication date October )Cited by: Most plant viruses, including PLYV, are good and effective antigens that, when artificially injected into a suitable warm-blooded animal, stimulate the production of specific antibodies that can be used in different serologic tests.
Rabbits are commonly chosen for the production of polyclonal antiserum because they are easily housed and adapt. Detection of viruses at early stages of infection is crucial to reduce economic losses. Biological indexing and serological enzyme-linked immunosorbent assay (ELISA) methods are widely used for diagnosis.
Nevertheless, molecular techniques. Although plant viruses do not have an immediate impact on humans to the extent that human viruses do, the damage they do to food supplies has a significant indi-rect effect. The study of plant viruses has led the overall understanding of viruses in many aspects.
HISTORY Although many early written and pictorial records of diseases caused by File Size: 1MB. The major vectors of plant viruses are: Insects. This forms the largest and most significant vector group and particularly includes: Aphids: transmit viruses from many different genera, including Potyvirus, Cucumovirus and picture shows the green peach aphid Myzus persicae, the vector of many plant viruses, including Potato virus Y.
Antigenicity of plant viruses Antigenically active part of a plant virus is protein except few viruses (external part of protein shells which encloses nucleic acid). Infectivity test depend upon nucleic acid only but serological test depend on its protein though these two occurs together or separate, but differ in their stability.
When it comes to serological properties, however, the similarities between animal, insect, bacterial, and plant viruses are paramount. The same immunochemical principles govern the in vitro serological reactions of all viral antigens, and much of general interest can be learned from the findings obtained with each particular group of by: 7.
So far, serological and nucleic acid-based assays are the most widely used methods for detection methods of plant viruses (Jeong et al., ; Ji et al., ;Yao et al., ). However, these. Cultivation of Viruses. Viruses can be grown in vivo (within a whole living organism, plant, or animal) or in vitro (outside a living organism in cells in an artificial environment, such as a test tube, cell culture flask, or agar plate).Bacteriophages can be grown in the presence of a dense layer of bacteria (also called a bacterial lawn) grown in a % soft agar in a Petri Author: Anh-Hue Thi Tu, Nina Parker, Mark Schneegurt, Brian Forster, Philip Lister.
Virology Techniques Introduction Virology is a field within microbiology that encom-passes the study of viruses and the diseases they cause. In the laboratory, viruses have served as useful tools to better understand cellular mechanisms. The purpose of this lesson is to provide a general overview of laboratory techniques used in the File Size: 2MB.
COVID Resources. Reliable information about the coronavirus (COVID) is available from the World Health Organization (current situation, international travel).Numerous and frequently-updated resource results are available from this ’s WebJunction has pulled together information and resources to assist library staff as they consider how to handle .In cases where the plant pathogen is difficult or impossible to grow on artificial media, other methods may be used for their detection, such as the use of serological tests for viruses.
Viral identification is often accomplished utilizing ELISA (enzyme-linked immunosorbent assay) which is based on the binding of an antibody produced to a. Free Online Library: Detection of Grapevine Fanleaf Virus Using Serological and Biological Assays.(Report) by "Journal of Basic & Applied Sciences"; Science and technology, general Bioassay Methods Biological assay Grapes Diseases and pests Plant viruses Health aspects Identification and classification Plant-pathogen relationships Virus diseases of plants.